Endometriome
The importance of assessing the endometrial microbiome
The balance of bacteria in the endometrium is a key factor for successful embryo implantation. In normal conditions, in the endometrium are mainly present different bacterial species of the Lactobacillus genus. The presence of dysbiotic or pathogenic bacteria may alter the endometrial microbiome and can disrupt the uterine environment, causing implantation failure and pregnancy loss.
Pathogenic bacteria: Staphylococcus, Streptococcus, Enterococcus, Mycoplasma, Ureaplasma, Enterobacteria (Escherichia, Klebsiella), Chlamydia and Neisseria. These bacteria cause infection, which is linked to implantation failure and recurrent miscarriage.
Dysbiotic bacteria: Bifidobacterium, Prevotella, Sneathia, Atopobium, Veillonella.Microbial imbalance is linked to embryo implantation failure.
Optimal microbiome: Lactobacillus.A balanced microbiome improves the reproductive prognosis, resulting in increased chance of pregnancy and live births Optimal.
A Complete View of the Endometrial Bacterial Composition
Endometriome™ - Endometrial Microbiome Analysis
As a new dimension of endometrial assessment to improve reproductive outcomes, Endometriome™ screening test can be applied.
Endometriome™ is a screening test that evaluates the endometrial microbiome to improve the clinical management of infertile patients. The Endometriome™ test provides a complete view of the bacterial composition of the endometrium, reports the 10 most common bacteria in the endometrium, and identifies the 8 most common pathogens that cause chronic endometritis (CE).
The Endometriome™ test can determine whether the uterine microbial environment is optimal for embryo implantation. Depending on the results, embryo transfer is recommended or, if necessary, antibiotic and probiotic therapy is prescribed to restore an optimal microbiome. The Endometriome™ test also detects bacteria-causing chronic endometritis and helps clinicians recommend appropriate antibiotic and probiotic treatments.
Endometriome™ test: can be applied to assess the microbiological environment that the embryo will encounter in patients with recurrent insemination failure (RIF) or recurrent pregnancy loss (RPL) or any patient wishing to conceive.
How is the Endometriome™ test performed?
DNA extraction is provided by taking an endometrial tissue biopsy or endometrial fluid from the sample taken. The Endometriome™ test can be performed between days 15 and 25 of the natural cycle or during the uterine secretion phase of an HRT cycle.
State-of-the-art Next Generation Sequencing (NGS) analysis screens 7 hypervariable regions (V2, V3, V4, V6, V7, V8 and V9) of bacterial 16S ribosomal RNA.
The report provides information on the microbial environment of the endometrial microbiome and recommends personalized treatment. This information includes the percentage of Lactobacilli in the Endometrial sample, the percentages of bacteria most represented in the Endometrial sample, whether the Endometrial biome is normal or abnormal, the detection of specific bacteria causing CE, and the detection and percentages of pathogens associated with sexually transmitted infections (Chlamydia and Neisseria spp).
RESULTS
Endometriome™ test results are determined in as little as 10 days. The results can be as follows;
POSITIVE RESULTS
Identification of dysbiotic or pathogenic bacteria with non-Lactobacillus dominant (<90%) endometrial microbiota and detects specific bacteria causing CE. It also determines the detection and percentages of pathogens associated with sexually transmitted infections (Chlamydia and Neisseria spp). This test result is associated with adverse reproductive outcomes (decreased vaccination success rate and significantly associated with increased miscarriage rate).
NEGATIVE RESULTS
Endometrial microbiome results are normal. (Lactobacillus predominant endometrium contains a high proportion of Lactobacilli, ≥90%.) Personalized treatment methods according to negative results can be; in the form of antibiotic therapy or probiotic therapy containing Lactobacillus (to ensure optimal microbiome) or embryo transfer for a suitable microbiome.